| 陸生
| R03 王燊琪 Shen-Chi Wang |
| 系所
| 臺灣大學植物科學研究所學位論文 |
| 指導教授
| 吳克強 |
| 中文題名
| 組蛋白甲基化轉移酶SUVH4，SUVH5和SUVH6與HDA6共同調控轉座子 |
| 英文題名
| The histone methyltransferases SUVH4, SUVH5 and SUVH6 act in concert with HDA6 to regulate transposons |
| 中文關鍵字
| 阿拉伯芥;組蛋白甲基化轉移酶;組蛋白去乙醯化酶;轉座子 |
| 英文關鍵字
| Arabidopsis;histone methyltransferases;histone deacetylases;transposons |
| 中文摘要
| SUVH4, SUVH5和SUVH6(SUVH4/5/6)是組蛋白H3賴氨酸9(H3K9)甲基轉移酶，都屬於SET結構域蛋白中SUV(R)亞群。我們發現SUVH4、 SUVH5以及SUVH6都位於細胞核內，並且可以與組蛋白去乙醯化酶HDA6相互作用。通過酵母雙雜交試驗我們發現HDA6的C端及SUVH5的C端對於他們之間的相互作用有關鍵的作用。此外，HDA6的絲氨酸S427的磷酸化會影響它與SUVH5/6的相互作用。通過對RNA-seq的資料分析，我們發現有許多轉座子的表達在hda6-6和suvh4 suvh5 suvh6 （suvh456）的突變體中都有上調，表明SUVH4/5/6和HDA6會共同調控轉座子表達。此外，在had6-6 及suvh456三重突變體中，組蛋白乙醯化水準上升了，而組蛋白H3K9的甲基化水準下降了。同時我們發現在hda6-6 及suvh456三重突變體中，轉座子AT5G59620和AT2G26630的H3K9二甲基化水準下降了。這些結果表明SUVH4/5/6可以通過與HDA6互作改變轉座子的組蛋白甲基化和乙醯化狀態，調控轉座子沉默。 |
| 英文摘要
| SUVH4, SUVH5 and SUVH6 (SUVH4/5/6) are histone H3 lysine 9 methyltransferases belonging to the SUV(R) group of SET domain proteins. I found that SUVH4, SUVH5 and SUVH6 are all localized in the nucleus and interact with the histone deacetylase HDA6. Both the C-terminal domains of HDA6 and SUVH5 are responsible for their interaction. In addition, phosphorylation on S427 of HDA6 may play an important role for the interaction of HDA6 and SUVH5/6. By the RNA-seq data analysis, we found that a number of transposons are up-regulated in both hda6-6 and suvh4 suvh5 suvh6 (suvh456) mutants, indicating that SUVH4/5/6 and HDA6 act in concert to regulate transposons. Furthermore, the level of histone acetylation is increased but the level of H3 K9 methylation is decreased in hda6-6 and suvh456 triple mutants. The level of H3K9Me2 in the transposons AT5G59620 and AT2G26630 is also decreased in hda6-6 and suvh456. Taken together, these results indicated that HDA6 and SUVH4/5/6 function together to regulate transposon silencing. |
| 出版日期
| 2016-08-03 |
| DOI
| 10.6342/NTU201601012 |
| DocID
| U0001-1807201612085200 |
